LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2174..2270) /label=attL2 /note="attL2 (c)" misc_signal complement(2285..2304) /note="T7 Promoter/priming site (c)" primer_bind 2312..2328 /note="M13 Reverse priming site" gene 2441..3250 /note="Kanamycin resistance gene" rep_origin 3371..4044 /note="pUC origin" PCR_frame 669..2174 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2194) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2191) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2182..4047,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2152..2172 /note="TEV site" source 673..2151 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:8856 IMAGE:3890907" /tissue_type="Lung, carcinoma, large cell undifferentiated." /clone_lib="NIH_MGC_69" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..2151 /gene="PIGV" /gene_synonym=FLJ20477 /gene_synonym=RP1-50O24.2 /db_xref="GeneID:55650" /db_xref="HGNC:26031" CDS 673..2151 /dnas_title="phosphatidylinositol glycan anchor biosynthesis, class V" /gene="PIGV" /gene_synonym=FLJ20477 /gene_synonym=RP1-50O24.2 /codon_start=1 /product="phosphatidylinositol glycan anchor biosynthesis, class V" /protein_id="AAH13568.1" /db_xref="GI:15488876" /db_xref="GeneID:55650" /db_xref="HGNC:26031" /translation="MWPQDPSRKEVLRFAVSCRILTLMLQALFNAIIPDHHAEAFSPP RLAPSGFVDQLVEGLLGGLSHWDAEHFLFIAEHGYLYEHNFAFFPGFPLALLVGTELL RPLRGLLSLRSCLLISVASLNFLFFMLAAVALHDLGCLVLHCPHQSFYAALLFCLSPA NVFLAAGYSEALFALLTFSAMGQLERGRVWTSVLLFAFATGVRSNGLVSVGFLMHSQC QGFFSSLTMLNPLRQLFKLMASLFLSVFTLGLPFALFQYYAYTQFCLPGSARPIPEPL VQLAVDKGYRIAEGNEPPWCFWDVPLIYSYIQDVYWNVGFLKYYELKQVPNFLLAAPV AILVAWATWTYVTTHPWLCLTLGLQRSKNNKTLEKPDLGFLSPQVFVYVVHAAVLLLF GGLCMHVQVLTRFLGSSTPIMYWFPAHLLQDQEPLLRSLKTVPWKPLAEDSPPGQKVP RNPIMGLLYHWKTCSPVTRYILGYFLTYWLLGLLLHCNFLPWT" misc_feature 673..2151 /note="PIGV coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgtggcc ccaggaccca tcccggaagg aggtgctgag gtttgcagtc 721 agctgccgta tcctgactct gatgctgcag gccctcttca atgccatcat cccagatcac 781 catgcagaag ccttctctcc tcctcgcctg gccccctcag gctttgtgga ccaactcgtg 841 gaaggtcttc tgggcggcct gtctcactgg gatgctgaac acttcttgtt cattgctgag 901 catggctacc tgtatgagca caactttgcc ttctttcctg gtttcccctt ggccctgctg 961 gtggggactg aactgttgag acccttacgg gggttactga gtctacgcag ttgcctgctg 1021 atttcggtag catcactcaa tttcttgttc ttcatgttgg ctgcagttgc acttcatgac 1081 ctgggttgtc tggttttgca ctgtccccac cagtcctttt atgcagctct gcttttctgt 1141 ctcagccctg ccaatgtctt cctggcagct ggttactcag aagctttgtt tgccctcctg 1201 acattcagtg ccatggggca gctggagagg ggccgagtct ggactagtgt actcctcttt 1261 gcctttgcca ctggggtacg ctccaacggg ctggtcagtg ttggcttcct catgcattct 1321 caatgccaag gctttttctc ttctctaacg atgctgaatc ctctgagaca gctctttaag 1381 ctgatggcct ctctgtttct gtcggtgttc acacttggcc ttccctttgc cctctttcag 1441 tattatgcct acacccaatt ctgtctgcca ggctcagccc gccccattcc tgagcctttg 1501 gtacagttag ctgtagacaa gggctaccgg attgcagagg gaaatgaacc gccttggtgc 1561 ttctgggatg ttccactaat atacagctat atccaggatg tctactggaa tgttggcttt 1621 ttgaaatact atgagctcaa gcaggtgccc aattttctac tggctgcacc agtggctata 1681 ctggttgcct gggcaacttg gacatacgtg accactcacc cttggctctg ccttacactt 1741 gggctgcaaa ggagcaagaa caataagacc ctagagaagc ccgatcttgg attcctcagt 1801 cctcaggtgt ttgtgtacgt ggtccacgct gcagtgctgc tgctgtttgg aggtctgtgc 1861 atgcatgttc aggttctcac caggtttttg ggctcctcca ctcctattat gtactggttt 1921 ccagctcact tgcttcagga tcaagagccg ctgttgagat ccttaaagac tgtgccttgg 1981 aagcctcttg cagaggactc cccaccagga caaaaggtcc ccagaaatcc tatcatggga 2041 cttttgtatc actggaaaac ctgttctcca gtcacacgat acattctagg ctacttcctg 2101 acttactggc tcctgggact actcctacat tgcaacttcc tgccttggac agaaaacttg 2161 tactttcaag gctCCAGCTT TCTTGTACAA AGTTGGCATT ATAAGAAAGC ATTGCTTATC 2221 AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA ATCATTATTT GCCATCCAGC 2281 TGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT GTTTCCTGGC AGCTCTGGCC 2341 CGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA ATAATATCAT CATGAACAAT 2401 AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA 2461 AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC 2521 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC 2581 GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT 2641 GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG 2701 TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT 2761 ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG 2821 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT 2881 CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA 2941 GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC 3001 ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG 3061 GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT 3121 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA 3181 AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA 3241 GTTTTTCTAA TCAGAATTGG TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG 3301 ACGGGACGGC GCAAGCTCAT GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG 3361 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT 3421 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA 3481 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC 3541 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC 3601 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT 3661 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG 3721 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA 3781 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT 3841 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA 3901 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC 3961 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 4021 CTTTTGCTGG CCTTTTGCTC ACATGTT //